Coding

Part:BBa_K4423008:Design

Designed by: Julia Vogl   Group: iGEM22_BOKU-Vienna   (2022-10-12)


Coding sequence for the gelatin protein.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1079
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1079
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1296
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1079
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1079
    Illegal NgoMIV site found at 262
    Illegal NgoMIV site found at 388
    Illegal NgoMIV site found at 424
    Illegal NgoMIV site found at 721
    Illegal NgoMIV site found at 1153
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gelatin coding sequence can not be syntesized in one piece due to a high number of repeats.


Source

The sequence of this part was taken out of the paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6787575/pdf/jfb-10-00039.pdf

References